Journal: International Journal of Molecular Sciences

Article Title: Regulated Mesenchymal Stem Cells Mediated Colon Cancer Therapy Assessed by Reporter Gene Based Optical Imaging

doi: 10.3390/ijms19041002

Figure Lengend Snippet: Bystander effect of MSC-Tet-TK and MSC-TK cells. ( A ) Rluc activity in co-cultures (1:1) of naive MSCs and CT26/Rluc cells treated with the indicated concentrations of GCV for 48 h. ( B ) BLI images of the Rluc activity and quantitation data of CT26/Rluc in co-cultures (1:1) of MSC-TK or MSC-Tet-TK cells in the absence or presence of doxycycline (DOX(−) and DOX 2 μg/mL respectively). Three individual experiment values are expressed as the mean ± standard deviation (SD), * p < 0.05, ** p < 0.01, *** p < 0.001 (by Student’s t test). p/s, photons/second.

Article Snippet: CT26 cells were transduced with lentiviral particles expressing mCherry-Rluc (Renilla luciferase) under the control of the cytomegalovirus (CMV) promoter (Genecopoeia, Rockville, MD, USA).

Techniques: Activity Assay, Quantitation Assay, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Regulated Mesenchymal Stem Cells Mediated Colon Cancer Therapy Assessed by Reporter Gene Based Optical Imaging

doi: 10.3390/ijms19041002

Figure Lengend Snippet: In vivo therapeutic effect of MSC-Tet-TK and MSC-TK cells on inhibiting colon tumor growth. ( A ) Renilla luciferase (Rluc) imaging of colon cancer cells (CT26/Rluc) in mice treated with either MSC-TK or MSC-Tet-TK cells with or without concurrent GCV treatment. BLI images were taken on days 0, 6, and 13 in five individual mice; ( B ) Quantitative analysis of the data shown in ( A ). ( C ) Tumor weights assessed at study end. Bioluminescence activity is shown in photons/second (p/s). * p < 0.05 compared separately to MSC-Tet-TK (GCV−) and MSC-TK (GCV−).

Article Snippet: CT26 cells were transduced with lentiviral particles expressing mCherry-Rluc (Renilla luciferase) under the control of the cytomegalovirus (CMV) promoter (Genecopoeia, Rockville, MD, USA).

Techniques: In Vivo, Luciferase, Imaging, Activity Assay

Journal: Frontiers in Veterinary Science

Article Title: Detection of bimodal survivin expressions in canine cancer types by flow cytometry compared to immunohistochemistry

doi: 10.3389/fvets.2025.1552415

Figure Lengend Snippet: Analysis of survivin expression sites by IHC and WB. (A) Images of HE staining and survivin expression via IHC in canine melanoma cell lines (CMM2, CMeC, and LMeC) are shown. (B) Images of HE staining and survivin expression via IHC in murine cell lines (p815, CT26, and B16F10) are shown. (C) The cytosol and total survivin expression patterns via WB are indicated. (D) The expression levels of total survivin (blue bar) and cytosolic survivin (orange bar) corrected on the basis of β-actin expression are shown via density of plot analysis via ImageJ software.

Article Snippet: A total of six cell lines were used: canine malignant melanoma lines [CMM2, CMeC2, LMeC; provided by Dr. Takayuki Nakagawa, Department of Veterinary Surgery, University of Tokyo; ( )], the murine malignant melanoma line B16F10, the murine mast cell tumor line p815, and the murine colon cancer line CT26 (distributed for a fee by the JCRB Cell Bank).

Techniques: Expressing, Staining, Software

Journal: Cell Reports Medicine

Article Title: CD8 + T cell-intrinsic IL-6 signaling promotes resistance to anti-PD-L1 immunotherapy

doi: 10.1016/j.xcrm.2022.100878

Figure Lengend Snippet:

Article Snippet: CT26 , Genentech , N/A.

Techniques: Control, Recombinant, Cell Stimulation, Staining, Cell Isolation, cDNA Synthesis, Expressing, CRISPR, Negative Control, In Situ Hybridization, Software